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Density increases as temperature decreases Density increases as salinity and pressure (depth) increase A typical profile consists of three layers: surface mixed layer, zone with rapid change in the variable being measured known as the thermocline (temperature), halocline (salinity) or pycnocline (density), and the deep mixed layer The salinity and temperature of seawater are determined when a water mass is at the surface and in contact with the atmosphere

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A change in a methyl-donor pool is not automatically beneficial and may vary between tissues