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In brief, exosome pellets were resuspended in 100 l of 50 mM Tris-HCl (pH 8.0) and incubated with 100 L of the Amplex Red reagent containing 2 U/mL HRP, 0.2 U/mL choline oxidase, and 100 M acetylcholine in a final volume of 200 L

The study population was recruited from a single center, which may limit the generalizability of our findings

Plozasiran is already approved as Redemplo in the US, EU, China, Australia, and Canada for familial chylomicronemia syndrome

In addition, genetic defects in folate metabolism and its transport can cause megaloblastic anemia and a variety of neurological disorders, including developmental delays, intellectual disability, seizures, and ataxia, underscoring the vital role of folate in brain development and function during both fetal and postnatal stages [28, 29]