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Description

Serrano Sandoval, Mauricio Graciano-Palacios, Janet A

doi: 10.1038/s41577-023-00894-6 155 TanakaAItoTKibataKInagaki-KatashibaNAmuroHNishizawaTet al

Isobaric labeling of digested peptides was carried out with TMT-10Plex (Thermo Fisher Scientific) reagent followed by MCX and SPE cleanup with appropriate buffer exchanges, and offline fractionation on Shimadzu Prominence with Xbridge 150 2.1 mm column (Waters) with two-minute fractions at a flow rate of 200L/min, and peptide amounts of 15mAU-equivalent aliquots from fractions 738 were concatenated