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Materials and methods Antibodies and reagents -Elemene (molecular formula C15H24), whose molecular weight is 204.35, was provided by Jingang Pharmaceuticals (#081152, Dalian, China)

Large-scale capture of peptides containing reversibly oxidized cysteines by thiol-disulfide exchange applied to the myocardial redox proteome

Finally, both acetylcholine -involved in learning and memory [73]- and DHEA-S-having anti-inflammatory and neurotrophic properties [74]- were increased in aged xCT / mice, reiterating improved hippocampal function when mice age in the absence of xCT [73,74,75]

Cells were then stained with PI prior to acquisition of 40,000 cells per tube, excluding doublets using the FACSCanto II flow cytometer