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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)

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On the northern half, Tumaccori National Historical Park, near the small community of Carmen, now encompasses 360 acres, while Tubac has evolved into a thriving arts community