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An air-cooled solid-state laser with an excitation wavelength of 532 nm was used to excite the samples

Importantly, both N-terminal FLAG-tagged and untagged WT IL-1 expressed in 293T cells were turned over at comparable rates to endogenous IL-1 in macrophages, and were degraded between 2 and 7 h following exposure to the protein synthesis inhibitor CHX (Figs