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Cell pellets were resuspended in 0.2 mL of buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0) for fluorescence measurement using a black 96-well plate in a SpectraMax M2e plate reader (Molecular Devices, San Jose, CA, USA) with excitation/emission wavelength pair of 488/512 nm

While both conjugates showed cytotoxicity in isolated rat renal proximal tubular cells, cytotoxicity was reduced for OPMA but not for its S -oxide when the cells were treated with methimazole, an inhibitor of flavin-containing monooxygenase (FMO)

Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation

doi: 10.3945/an.114.006874