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Brian Kuhlman, University of North Carolina, Chapel Hill |Computer-Based Design of Protein Complexes and Conformational Switches

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Materials and methods Generation and primary characterizations of SCC-61/rSCC-61 matched model system of radiation resistance in HNSCC We have reported the establishment of the radiation resistant rSCC-61 cell line in a previous publication ( Quantitative redox proteomics SCC-61 cells and rSCC-61 cells were cultured in DMEM/F12 media containing the light and heavy isotopes of Lys8 and Arg10, respectively, and supplemented with 10% dialyzed FBS and 200 mg/L proline to prevent the conversion of isotope-coded arginine to proline in cells ( HPLC fractionation Basic high pH reversed-phase chromatography was conducted on a Waters 2695 instrument using a Xbridge C18, 3.5 m, 4.6 x 150 mm chromatography column