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Although dietary lipids encompass triacylglycerols, phospholipids, sterols (predominantly cholesterol with minor phytosterols), and small amounts of free fatty acids 10,11 only fatty acyl chains are oxidizable for yielding energy, whereas cholesterol is catabolized chiefly to bile acids rather than used as an energy substrate 12

These results demonstrate that NF1 exon skipping-induced restoration of NF1 function enhances the anticancer efficacy of Paxalisib through dual PI3K/mTOR inhibition and apoptosis induction, validating the synergistic efficacy and safety of the combination in both the patient-specific in vivo model and the NOCS platform

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Apoptosis is finally triggered by this mechanism, which results in glutamine suppression of mTORC1 signaling targets ( However, glutamine is frequently competitively and malignantly absorbed by tumor cells in the TME, which prevents T cell differentiation