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Western blot analysis Total protein extracts from liver tissues and HepG2 cells were prepared using RIPA buffer containing 50 mM Tris-HCl, 150 mM NaCl, 5 mM MgCl2, 2 mM EDTA, 1 mM NaF, 1% NP40, and 0.1% SDS

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Some of the characteristic symptoms of this deficiency include: extreme tiredness lack of energy breathlessness feeling faint headaches pale skin noticeable heartbeats (palpitations) loss of appetite and weight loss a decline in your mental abilities (brain fog) irritability depression If youre following a vegan diet and recognise any of the symptoms outlined above (or just wish to prevent them!), a vegan B12 supplement may be required and you have two options available

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