glutathione agarose sigma g4510 Millipore High Capacity Magnetic Beads, (1:1 suspension in a 30% ethanol solution) 1 ml molecular pull Protino Glutathione Agarose 4B for

Some studies indicate DSIP does not induce pharmacological tolerance with continued use [2]

Needle: 30G or 31G, 6 mm fixed needle insulin syringe

Wash hands thoroughly Clean the tops of both vials with alcohol swabs Draw your chosen solvent into a sterile syringe Inject the solvent slowly into the IGF-1 LR3 vial, directing the stream against the glass wall rather than onto the powder Let the vial sit undisturbed for several minutes, allowing the peptide to dissolve naturally Gently swirl to mix, never shake vigorously Confirm the solution is clear with no visible particles Label with date and concentration For a 1 mg vial, adding 1 mL of solvent creates a concentration of 1000 mcg/mL

REC tags are suitable for proximity-dependent biotinylation experiments Next, we examined whether the REC tag was detectable after proximity labelling of cells using TurboID