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After several washes in the same buffer, the samples were post-fixed for 60 minutes with 2% osmium tetroxide, washed in deionised water, dehydrated through graded ethanol (70, 95, and 100%) and embedded in epon for 48 hours at 60 C

Edwards R, Dixon DP: Plant glutathione transferases

Partial correlation analysis investigated the relationship between changes in these brain outcome measures and PTSD symptom severity, considering age and sex as covariates