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In brief, the pyocyanin-ascorbic acid mixture was mixed with chloroform (Univar., Ingleburn, Australia) to a dilution factor of 300 L of chloroform for every 1,000 L of the mixture in 15 mL falcon tubes and vigorously shaken manually and vortexed immediately for 5 s followed by centrifugation at 4500 g for 10 min at 4C

longa may be attributed to curcuminoids, including curcumin, demethoxycurcumin, bisdemethoxycurcumin, and cyclocurcumin, present in approximate proportions of 70%, 17%, 3%, and 10%, respectively (Trujillo et al., 2013

Interestingly, several Rab GTPases were only observed in the oleic acid condition

Sure enough, when the researchers were using this product and getting positive responses in the immune responses for the patients