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The cells were then lysed in RIP lysis buffer containing protease inhibitor cocktail (PIC) (Beyotime, Shanghai, China) and phenylmethylsulfonyl fluoride (PMSF) (Beyotime, Shanghai, China) on ice for 30 min, followed by centrifugation at 13,000 rpm for 15 min at 4

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In this article, well discuss what exactly glutathione agarose is, and how its used in protein purifications and for pulldown experiments

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