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Methods: Our study utilized C57BL/6 mice models for hepatic fibrosis induced by both CCl4 administration and a high-fat diet, mimicking metabolic dysfunction-associated steatohepatitis (MASH)

Yohimbine is studied in alpha-2 receptor and catecholamine-response research, while Clen is studied in beta-adrenergic pathway models involving cellular energy response, thermogenic signaling, and metabolic regulation

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Because the bound copper is integral to the molecule's identity, the mass measured by LC-MS reflects the intact complex, confirming that the copper coordination has been retained through synthesis and purification