L- 1-2 (): 7:00 - 250 30 - 500 : 3-8 ( ): 7:00 - 500 30 - 1000 : 500 + 250 : 40-60 + : 7:00 - 750 45 - 1000 - 500 ( ) : 7:00 - 1000 30 - 1500 : 750 + 500 : : 7:00 - 300 13:00 - 200 : 2 , 1 : 7:00 - 500 19:00 - 250 3 : L- : 30 30-40 200 2-3 2-4
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DL-carnitine (20mg) was weighted precisely and put in 100mL volumetric flask and dissolved with water which is added to volume as well
We amplified promoter regions (of rab3 , trx1 , oig1 , osm3 , glr1 , gpa9 ) to allow us to express dsRNA in a tissuespecific manner (nervous system, ASJ, IL2, PVQ neurons, respectively) (Appendix Fig S8, Appendix Table S5)