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Quercetin-induced pyroptosis in colon cancer through NEK7-mediated NLRP3 inflammasome-GSDMD signaling pathway activation

This study is performed in accordance with relevant guidelines and regulations

Paneco, 135), while the other part was seeded on plastic plates (Corning) and cultured for no longer than 8 passages in RPMI growth medium (Paneco, 330) supplemented with 10% FBS, 2 mM glutamine (Gibco, #35050061), 1% penicillin/streptomycin (Paneco, A065), and 1% non-essential amino acids (Paneco, 115/100)