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With inputs of experimental isotopologue patterns for metabolite substrates, we used the joint probability theory to predict isotopologue patterns for the specific metabolite of interest

For immunostaining of MBP and neurofilament-200 (NF-200), after fixation of the cells with 4% PFA, the cultures were treated with 0.3% Triton X-100 in PBS for 30 min and incubated with primary antibodies (Table 2) in PBS containing 1% HS overnight at 4 C

It is expressed in zygotes and early embryos and presents both in the cytoplasm and nuclei of embryos

The human gut sterolbiome: bile acid-microbiome endocrine aspects and therapeutics