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Primarily, Botox injections target the nerve endings in the muscles, blocking the release of neurotransmitters that carry pain signals to the brain

Subcutaneous injection in the vicinity of an injured tendon or muscle provides both local and systemic effects, maximizing exposure where healing is most needed while still distributing the compound throughout the body

We screened candidate mutant lines by PCR and Sanger sequencing and identified the Atg8a 12 allele that carries an 8-bp deletion based on sequencing data (Fig S2A), which causes frameshift in all isoforms of the Atg8a gene

However, this complex surgical procedure is associated with significant postoperative morbidity and mortality