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At the start of differentiation (day 0), B8 medium was changed to CDM3 (chemically defined medium, three components) (Burridge et al., 2014), consisting of RPMI 1640 (Corning, 10-040-CM), 500 g ml 1 fatty acid-free bovine serum albumin (GenDEPOT), and 200 g ml 1 L-ascorbic acid 2-phosphate (Wako)