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In brief, EIA microplates (Corning CLS3590) were coated overnight with 100 l of coating buffer (carbonate-bicarbonate with pH = 9.6, Sigma C3041) or phage Muddy diluted to 5 10 9 pfu ml 1 in coating buffer

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Shelf life: Several years when properly stored at -20 degrees Celsius Reconstituted (liquid) form: Temperature: Refrigerate at 2 to 8 degrees Celsius immediately after reconstitution Shelf life: Use within 2 to 4 weeks for optimal potency Never freeze reconstituted peptides Do not expose to repeated temperature cycling For comprehensive storage guidance, our articles on how to store peptides after reconstitution , how long reconstituted peptides last in the fridge , and general peptide fridge storage guidelines provide detailed protocols for maintaining peptide integrity