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Endotoxin entry into cells occurs via mechanisms dependent on plasma membrane structures such as lipid rafts and clathrin-dependent mechanisms [48]

The resulting lysates were incubated with either PBS or 3-HK (5 M) for 60 min at room temperature

Similar to previous protocol, we carried out tandem affinity purification and analyzed the protein complexes using negative stain electron microscopy, which exhibits a comparable dimeric architecture (Supplementary Fig