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[DOI] [PMC free article] [PubMed] [Google Scholar] 191.Bosco A.M., Almeida B.F.M., Pereira P.P., Dos Santos D.B., Neto .J.S., Ferreira W.L., Ciarlini P.C

Vitamin D metabolism is mainly regulated by parathyroid hormone (PTH), produced by the parathyroid glands, and fibroblast growth factor 23 (FGF23), synthesized by osteoblasts and osteoclasts

Columns were placed in 15 ml collection tubes and centrifuged three times at 1000 g for 2 min at 4 C with 1 ml of 150 mM potassium phosphate buffer (pH 8.0) per wash