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Chlorophyll fluorescence was measured using a fluorometer (Open FluorCam FC 800-O/2020, Photon Systems Instruments, Brno, Czech Republic) and F v /F m was analyzed based on the fluorescence image

Currently in clinical trials is a long-term naltrexone implant that slowly dissolves and continuously released the naltrexone dose for about six months

5-Amino-1MQ dosing protocols are less standardized due to the absence of clinical trials

Hence, OoCs are increasingly used to study disease mechanisms, discover biomarkers, and test therapeutic candidates with improved accuracy compared to traditional cell cultures or animal models ( Figure 1 )