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For kinetic analysis, absorbance at 340 nm was recorded using a multifunctional enzyme labeling instrument in kinetic mode over a period of 5 min, with measurements taken at 6-s intervals

The combination of mod-1 rescue in RIC neurons and aversion in mod-1 tbh-1 double mutants indicates that octopamine from RIC can suppress aversion from the cysE diet, but is inhibited by mod-1

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For LC-HRMS/MS analysis, the supernatant was centrifuged, evaporated to dryness, and resulting pellets were reconstituted in mobile phase MPA2 and MPB2 (90:10, v/v)