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Firstly, long-term exposure to air pollutants (PM2.5, NO, ozone) increases intestinal permeability, systemic inflammation, and dysbiosis [221], The UK Biobank cohort has confirmed that PM2.5 and NO significantly increase the risk of UC [222], Other studies have also revealed a correlation between PM2.5 exposure and increased rates of intestinal resection and mortality [223]

Quantification of the percentage of Safranin-O positive areas was performed using ImageJ (version 1.53t, National Institutes of Health, Bethesda, MD, USA)

To get 1000mcg/mL: You will add exactly 1mL (or 100 units on an insulin syringe) of bacteriostatic water to the 1mg vial