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For protein expression, 800 mL HEK293F cells (R79007, Invitrogen) were cultured in SMM 293T-II medium (M293TII, Sino Biological Inc.) in a shaker, at 37 C, under 5% CO 2

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[22] [23] [24] Glioblastomas with a wild-type IDH1 gene have a median overall survival of only 1 year, whereas IDH1 -mutated glioblastoma patients have a median overall survival of over 2 years