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Samples were centrifuged at 16,000 g for 10 min at 4 C, and 10 l of supernatant was mixed with 90 l of the quenching reagent (0.5 mM -mercaptoethanol in water)

As a consequence of metal absorption from the soil, plants are more suspectable to oxidative stress, and special care is taken in preventing reaction between transition metals and H 2 O 2 by their sequestration

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Growth hormones are crucial for fat loss, as they increase the bodys ability to burn fat, especially when combined with muscle-building activity