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Treatment and control cells (5 mL) were cultured separately in 2YT media containing ampicillin (200 g/mL) and CAM (30 g/mL) at 37 C, 180 rpm

To analyze the importance of these three cysteines, we have generated GSNOR mutants, where these cysteine residues were exchanged by serine resulting GSNOR C47S , GSNOR C177S , and GSNOR C271S