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Fecal elimination of metabolites also occurs, particularly following oral administration, though quantitative contribution of each elimination pathway varies by route and dose

Briefly, amino acids in the hydrolyzed samples were derivatized by mixing 70 L of Waters AccQ-tag buffer with 10 L of the sample or standard and then 20 L of AccQ-tag reagent, followed by incubation at 55 C for 10 min