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Renin, a proteolytic enzyme secreted by the juxtaglomerular apparatus of the kidney, cleaves angiotensinogen at the N terminus to form the decapeptide, Ang I

Fructose-6-phosphate, the substrate of phosphofructokinase-1, is directly responsible for this glycosylation as it is metabolized to UDP-N-acetylglucosamine through the hexosamine biosynthetic pathway 104