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The GAPDH substrate 1,3 bisphosphoglycerate was generated by incubating 13.5 mM 3-phosphoglycerate, 6 mM ATP with 0.012 U/l 3-phosphoglycerate phosphokinase in 100 mM Tris/HCl p H 8.0, 1 mM EDTA, 15 mM MgCl 2 for 20 to 30 min at RT

10.1159/000501645 259 SchneiderI.WettsteinA.JenniG.KhneF.HoltforthM.WachsS.et al (2025)

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