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Cellular ROS detection assay Following spheroids treatment with different Vit-C doses for 72 h, the cellular ROS was determined by performing the ROS-GLO H 2 O 2 ASSAY (PROYY-G8820, Promega, USA) according to the protocol from the manufacturer

Recently, it was shown that lymphomas lacking TrxR1 can initiate and progress normally in mice (Mandal et al., 2010), adding another cell type to the list of cell types that can replicate in the absence of TrxR1

Data analyses All data are presented as mean SEM and were analyzed by one-way and two-way ANOVA followed by Bonferroni corrections for multiple comparisons between means using the error mean square terms from each ANOVA analysis as described previously 36

& Sziklai-Lszl, I.) (1998)