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This study employed a top-down protein MS analysis to identify the most reactive cysteine sites in MT2, confirming that both compounds induce S-thioallylation and alter Zn(II) binding

In contrast, the VAMP2-binding deficient mutant Munc18-1 Q301D lacked the stimulatory effect and fusion was reduced by 50% below the level obtained without Munc18-1 addition (Fig