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For the generation of the chimeric E1 (ChE1) gene, the gene encoding E1 (41-957) was fused to the 5-terminus of the gene encoding Sae2 (450-549), and the A680T, N728Y, F729I, A788S, D853E, and T898S mutations were generated by PCR 54

Specifically a different allele of the Human Leukocyte Antigen (HLA) gene