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However, an inhibition of RIP1K using necrostatin-1 or the knockdown of Ripk3 had no significant protection against DOX-induced cell death (Supplemental Figure 8, A and B), and Ripk3 knockdown suppressed DOX-induced caspase cleavage (Supplemental Figure 8C), suggesting that necroptosis through RIP3K is a relatively minor form of RCD under DOX treatment and might be recognized as a part of apoptosis

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Because Cys20 oxidizes naturally as well as artificially, the C20S mutant was used for structural determination of the complexes