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Symptoms of vitamin B12 deficiency include: Anemia Intestinal disorders and weight loss Fatigue, weakness, lightheadedness Memory loss or confusion Depression or dementia Rapid heartbeat and respiration Mouth or tongue soreness or bleeding Excessive bruising Injections are the most effective means of administration of vitamin B12 since they do not depend on the body's ability to absorb the substance through the gastrointestinal tract

The mixture was incubated at 37 C for 15 min with intermittent gentle vortexing and sonication to ensure uniform dispersion and association of nervonic acid with BSA

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