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On day 2, tissues were washed again using 0.1 M PBS, incubated in secondary antibody diluted by blocking solution for 2 h at room temperature, covered to prevent light exposure, washed again, and cover-slipped using DAPI Fluoromount G (Southern Biotech) or Fluoromount G (Southern Biotech)

SBP and DBP were significantly decreased by 19 and 12 mmHg, respectively (Verma et al., 2009)

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