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Limitations and realistic expectations

In the absence of Cur-AuNPs, no oxidation of TMB was observed, even in the presence of HO, indicating that the reaction is not spontaneous and confirming the necessity of a catalyst, similar to the action mechanism of horseradish peroxidase (HRP)

A trap-and-elute dual-column setup was used, with a PepMap Neo Trap Cartridge (300 m 5 mm, 5 m) serving as the trapping column, and an Easy-Spray PepMap Neo UHPLC column (150 m 15 cm, 2 m) as the analytical column

Alcohol consumption: Alcohol, particularly beer and liquor, can increase the risk of gout by interfering with the bodys ability to excrete uric acid