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Diglutathionylated wild type protease Cys-67-SSG, Cys-95-SSG monogluthionylated protease mutants C67A, Cys-95-SSG and C95A, Cys-67-SSG thioltransferase glutathione Cys-67-SSG was partially deglutathionylated but only at 20% of the rate of Cys-95-SSG reduction

Then samples were incubated with primary anti-MUC-1 (Servicebio) and anti--actin (Abclonal, Wuhan, Hubei, China) at 4C overnight

Another test, IgA endomysial antibodies, may be used to confirm positive results

However, relatively frequently, the diffuse histological and plexiform pattern coexist in subcutaneous and deep lesions,57 and so the clinical nomenclature does not always coincide with the histological characteristics