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The fluorescence signal from EGFP-positive cells remained detectable over the entire observation period, enabling longitudinal analysis of their localization and distribution

Zewinger et al., 2016)

The brain was quickly removed and placed in an oxygenated ice-cold cutting solution

It is well established that the function of TolC can be disrupted with small molecule inhibitors such as phenylalanine-arginine b-naphthylamide (PAN) 63