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Gibco) at 37 C and 5% carbon dioxide

Synthesis and identification of lithocholic acid 3-sulfate as RORt ligand to inhibit Th17 cell differentiation

Analysis was performed in negative ion mode at a resolution of 140,000 for the full MS scan in a data-dependent mode

We observed that EAAT3 knockdown, similar to NF-B inhibition, led to the accumulation of reactive oxygen species (ROS) and increased sensitivity to ferroptosis induction by RAS-selective lethal 3 (RSL3)