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For cell models in need of external metabolizing systems such as S9-mix, NAs are already activated extracellularly and the activated/reactive metabolites must cross both cell and nuclear membranes for DNA adduct formation

In the worst-case scenario, introducing contaminated solutions into a sensitive biological system can lead to wildly unpredictable outcomes, from cell culture death to inflammatory responses in in-vivo studies

To make this comparison even clearer, this table breaks down the fundamental differences between conventional approaches and our regenerative strategy for osteoarthritis