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Description

FLAG-tagged proteins were directly visualized using HRP-conjugated M2 anti-FLAG (Abcam, RRID: AB_869428) or indirectly by combining M2 anti-flag (Sigma-Aldrich catalog number F3165, RRID: AB_259529) and anti-mouse-HRP (Abcam catalog number ab6808, RRID: AB_955441)