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Stained cells were then washed once with PBS and ROS levels were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter) with lasers at wavelengths of 405 nm (violet), 488 nm (blue), 561 nm (yellow), and 633 nm (red)

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(2016) found that withaferin A is a strong inducer of Nrf2, a transcription factor that controls the production of antioxidant enzymes in response to oxidative stress through activation of the PTEN/PI3K/Akt pathway [146]