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For 3D cultures, Promega recommends CellTiter-Glo 3D Cell Viability Assay, which uses an enhanced lysis buffer specifically formulated to penetrate 3D tumor spheroid structures and release ATP from cells throughout the spheroid

coli hosts such as XL-1 Blue (Stratagene Cloning Systems, La Jolla, Calif.) cells are transformed with the ligation mixture and spread on culture plates

Glucocorticoid-induced osteopenia in the mouse as assessed by histomorphometry, microcomputed tomography, and biochemical markers

These effects are believed to be related to the broader systemic benefits of optimized GH circulation on brain function and hormonal balance