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Melatonin is an indolic compound with lipophilic properties derived from the amino acid tryptophan, which is taken up by pinealocytes from the peripheral circulation and transformed into melatonin through the action of four participating enzymes [Figure 1]

The selected particles were then subjected to reference-based motion correction in cryoSPARC (v4.5.0), followed by several rounds of heterogeneous refinement using volumes of different states of SLC33A1, resulting in a final stack of 215,071 particles

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