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Discussion Investigating the mechanisms underlying glaucoma pathogenesis have long been a research focus in ophthalmology

Tissue Preparation Organ tissue was fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h (brain, liver, kidney, lungs, heart, stomach, intestines)

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Equal amounts of protein lysates were loaded on Novex WedgeWell Tris-glycine or Bis-Tris gels (ThermoFisher) and blotted onto nitrocellulose or PVDF Trans-Blot Turbo membrane (0.2 m, Bio-Rad) according to standard protocols