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Most of the cases were treated with metformin (80.4%), either as monotherapy or associated to subcutaneous insulin treatment

Preparation of assay reagents Biotin-conjugated GX-G3 was prepared using SureLINK Chromophoric Biotin Labeling Kits from KPL Inc

To further characterize these interactions, we constructed a molecular interaction network (Figure 7C) revealed three functionally interconnected modules: ( Consistent with multi-omics findings, KEGG pathway analysis identified glutathione metabolism as the most significantly perturbed shared pathway (Figure 8A), exhibiting coordinated changes between upregulated antioxidant enzymes such as Gpx3 and Mgst1 alongside depleted glutathione derivatives, supporting redox imbalance as a key pathogenic feature

doi: 10.2147/NDT.S147897 8