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Antioxidant systems play a crucial role in preventing peroxidative damage by scavenging free radicals or indirectly consuming compounds that induce free radical generation, thus preventing ferroptosis

This minimal vector was linearised by PCR (Phusion TM Plus DNA Polymerase (ThermoFisher Scientific, #F630S) and gel purified

58 LuoY.WangS.-X.ZhouZ.-Q.WangZ.ZhangY.-G.ZhangY.et al

Melatonin induces an increase in the expression of p53 and p21/WAF, which act as negative regulators by inhibiting cyclin-dependent kinases and preventing the phosphorylation of the Rb (retinoblastoma protein), which is part of the last checkpoint of the G1 phase